Figure 9

Immunohistochemical labelling in the capybara AOB. (A) The IHC labelling with anti-Gαi2 stains the nervous and glomerular layers of the anterior area of the AOB. (B) On the other hand, the anti-Gαo marks all of the nervous tissue except the anterior part of the AOB, resulting in a complementary expression pattern of both G proteins. (C,D) Marking with anti-GFAP produces a more prominent diffuse pattern in the nervous and glomerular part of the AOB. (E,G) In the AOB, both anti-Calretinin (CR) and anti-Calbindin (CB) produce a complete label more intense in the glomerular layer (arrowheads). (F,H) MAP2 labelling focuses on the external plexiform and in the glomerular layers (arrowheads). (I,J) The anti-OMP is immunopositive in the MOB, marking intensely the nervous and glomerular layers (GlL) whereas in the AOB the labelling is very faint, except in the GlL (arrowheads). a: Anterior; p: posterior. Scale bars: (A–C,E–G and J) 500 µm; (D,H and I) 250 µm.