Table 3 Primer and probe sequences for the SNPs V410L, V1016I and F1534C kdr in Aedes aegypti.
From: Kdr genotyping in Aedes aegypti from Brazil on a nation-wide scale from 2017 to 2018
NaV site | Assay IDa | Variation | Primers | Probes |
|---|---|---|---|---|
410 | AN2XA9W | GTA/TTA | For: GTGGCACATGCTCTTCTTCATT | Val: VIC-TCGTTCTACCTTGTAAATT-NFQ |
(Val/Leu) | Rev: GGCGACAATGGCCAAGATC | Leu: FAM-TTCGTTCTACCTTTTAAATT-NFQ | ||
1016 | AHS1DL6 | GTA/ATA | For: CGTGCTAACCGACAAATTGTTTCC | Val: VIC-CCCGCACAGGTACTTA-FAM |
(Val/Ile) | Rev: GACAAAAGCAAGGCTAAGAAAAGGT | Ile: FAM-CCGCACAGATACTTA-NFQ | ||
1534 | AHWSL61 | TTC/TGC | For: TCGCGAGACCAACATCTACATG | Phe: VIC-AACGACCCGAAGATGA-NFQ |
(Phe/Cys) | Rev: GATGATGACACCGATGAACAGATTC | Cys: FAM-ACGACCCGACGATGA-NFQ |
