Figure 2
From: A chimeric mouse model to study human iPSC-derived neurons: the case of a truncating SHANK3 mutation
Integration of human iPSC-derived neurons into mouse brain ventricles and maturation pattern of axonal projections. (a) Camera lucida drawings of the pattern of axonal projections 50 days after transplantation within mouse ventricles. Abbreviations are according to Allen Brain Atlas (https://mouse.brain-map.org/). Image credit: Allen Institute. Neuron location, migration and densities are depicted using a code-color schematic representation. Results are from two independent mice, using 72 brain slices of 50 μm each. The illustrated data are from one representative brain. (b) Visualization of GFP-positive cells with their cell bodies, dendrites and axons (green color) within the ipsilateral ventricle. Structures from the olfactory system (Bregma 2.445 mm; 0.145 mm), lateral septal nucleus (Bregma 0.945 mm), a medial olfactory area, bed nuclei of the stria terminalis (Bregma 0.145 mm), a relay center composed of high densities of fibers, and piriform cortex (Bregma − 0.38mm), receiving back projections from olfactory bulb. Data illustrate the integration of human neurons in structures situated on the outside walls of the lateral ventricles, as well as their possible migration within the rostral migratory stream for integration within olfactory regions. No labeling was observed along the subgranular neurogenic zone of the dentate gyrus. A faint labeling was observed in corpus callosum and in some contralateral lateral areas (a). Scale bars = 50 μm. (aco) Anterior commissure, olfactory limb; (CP) Caudate putamen; (CTX) Cortex; (HIP) Hippocampal region; (OLF) Olfactory areas; (PAL) Pallidum; (VL) Lateral Ventricle; (3V) Third Ventricle (LS) Lateral septal nucleus; (Ipsi) Ipsilateral; (Contra) Contralateral.
