Figure 7
Quantitative real time PCR (qRT-PCR) analysis of relative transcript expression of 18 candidate genes encoding SsDCL, SsAGO, and SsRDR in S. spontaneum clone SES208 exposed to dehydration treatment (PEG-6000) for 0, 3, 6, and 12 h. The x-axis indicates the time points of PEG-6000 exposure, whereas the y-axis indicates the relative expression level. The top young leaves were sampled and used for qRT-PCR assay. Relative transcript expression values are presented as means ± standard errors based on three biological replicates with three technical replicates. Significant differential expression is indicated by an asterisk (*, p < 0.05; **, p < 0.01).
