Figure 1
From: Uncovering a possible role of reactive oxygen species in magnetogenetics
Magnetogenetic platform expressed in HEK-293T cells. (a, i–iii) Constructs used to express the magnetogenetic platform in HEK-293T cells. (a, i) Construct used to stably integrate the αGFPnb-TRPV1-T2A-eGFP-FtD platform and create a stable cell line for magnetogenetic stimulation studies. (a, ii) Construct used to transiently transfect the Ca2+-dependent SEAP reporter. (a, iii) Construct used to stably integrate the eGFP-FtD portion of platform and create a stable cell line for ferritin immunoprecipitation studies. (b) Cartoon schematic of a monomer of the αGFPnb-TRPV1 fusion conjugated to eGFP-tagged chimeric ferritin and activated through magnetic field stimulation to induce gating of calcium. (c) Brightfield and (d) fluorescent images of stably expressing nbV1/FtD cells showing expression and localization of the αGFPnb-TRPV1-T2A-eGFP-FtD construct (a, i) to cell membranes.
