Figure 1

MALDI-MS spectrum of N-glycans recorded from SDS-purified E glycoprotein of TBEV grown in human neuroblastoma cells (UKF-NB4). Glycans were detected immediately after incubation with PNGase F and neuraminidase applying on-target derivatization with PHN (+ 90.06 increase in mass and detected as MNa+ ions). No purification procedure was applied here.