Figure 3

MALDI-MS spectrum of N-glycans recorded from TBEV grown in tick cells (IRE/CTVM19). Sample underwent incubation with PNGase F and neuraminidase, following SPE purification. Glycans are labeled at the reducing termini with PHN, and detected as MNa+ ions. Peaks with an asterisk correspond to sialylated glycans producing additional multiply sodiated ions and are the result of incomplete desialylation. For more detailed information about detected N-glycans see Supplementary Table 1.