Figure 3
Comparison of experimental results and model simulations. (a) PI and Ins experimental results (orange dots with SD) and simulations (blue curves) after Thrombin stimulation. Secondary signaling by other, secreted, GPCR ligands is taken into account and assumed to happen immediately. (b) Early model iterations simulations showing the stabilizing impact of lipid binding proteins (BP) on the dynamics of PI45P2, PI4P and PA before and after activation with Thrombin. The numbers of BP and the ON/OFF rates of their binding to their relevant PL were determined using parameter scans. (c) The final simulations in our completed core model are shown together with the experimental results. Experimental results are shown with standard deviations when available. (References, SD and model parametisation procedures in “Methods” and Supplementary Tables S1-S5). Experimental data: n = 10, SEM are shown. Activation is indicated by arrow.
