Figure 7
Simulation results of systematic “mix-and-match” of protein numbers between HeLa and U2OS cells proteomic data. The copy numbers of the 12 key proteins surveyed in our model (Gαq, smG, PLCβ, the combined IP3 modifying enzymes, DGK, LPP, CDIPT, OCRL1, PI4K, PIP5K, SAC1 and cPLA2) were taken from either the HeLa or U2OS proteome (12, 34), or from the calculated numbers for U2OS missing data shown in supplementary Table S7, and systematically mixed using Parameter Scans in COPASI 15. 75% of the 4,096 simulations obtained lead to incorrect dynamic behaviours for the outputs monitored with our model. Simulations are run for 5,000 s with activation occurring at 1,000 s (arrows), #molecules per cell, s seconds.
