Figure 3

Current versus exposure time analysis. Examples of chloride current peaks evoked by 10 µM uncaged RuBi–GABA (V = − 80 mV) at different exposure times for 1PE (458 nm, 9.36 µW) (A) and 2PE (750 nm, 125 mW) (E) on the same cell. Normalized values of current peak evoked by 10 µM uncaged RuBi–GABA at different exposure times for 1PE (458 nm, 9.36 µW) (B) and 2PE (750 nm, 125 mW) (F), the values are normalized to the current measured for the lowest exposure time (1 ms). Time of decay as a function of the exposure time (τ) for 1PE (458 nm, 9.36 µW) (C) and 2PE (750 nm, 125 mW) (G). FWHM versus exposure time for 1PE (458 nm, 9.36 µW) (D) and 2PE (750 nm, 125 mW) (H). Each experimental value is estimated by calculating mean ± SEM on the data collected on at least 4 cells.