Figure 5
From: Metabolomics profiling reveals new aspects of dolichol biosynthesis in Plasmodium falciparum
PfPPRD restored N-glycosylation of mCPY and reduced levels of polyprenols (POH) in yeast dfg10Δ mutant cells. (a) Glycosylation status of CPY in dfg10Δ mutant cells expressing yeast DFG10 or PfPPRD. Positions of mature CPY (mCPY) and its glycoforms (− 1, − 2, − 3, − 4) are indicated. Empty vector (vector) was used as a control. (b) Extracted ion chromatogram (EIC) from LC-HRMS analyses of yeast strains showing that for WT only dolichol (DOH) of 15 and 16 isoprene units are detected while in dfg10Δ mutant strain dolichols are accompanied by their respective polyprenols 15 and 16. Yeast dfg10Δ mutant strain transformed with PfPPRD showed considerable reduction of polyprenols 15 and 16. Data presented are representative of three independent biological replicates. The figure was generated using CorelDraw 2018 (https://www.coreldraw.com/en/) and Adobe Photoshop 21.2.0 (https://www.adobe.com/products/photoshop.html).
