Figure 1

Human NPC1 Western blot of COS cell lysates removed 2 days following cell lipofection with Lipofectamine 2000 and 2 μg DNA per dish of either no plasmid (control), pPDGF-B-NPC1 plasmid DNA, or pCMV-NPC1 plasmid DNA. Duplicate lanes of each are shown. Lipofection with either NPC1 expression plasmid produces a 180–200 kDa immunoreactive protein, which corresponds with the size of the human NPC1 protein.