Figure 2 | Scientific Reports

Figure 2

From: Plasmid DNA gene therapy of the Niemann-Pick C1 mouse with transferrin receptor-targeted Trojan horse liposomes

Figure 2

ELISA results of binding of either unconjugated recombinant TfRMAb or the DSPE-PEG2000-TfRMAb conjugate. The latter is formed following solubilization of TfRMAb-conjugated THLs with 0.2% Triton X-100. The thiolated TfRMAb forms a thio-ether linkage at the maleimide tip of the DSPE-PEG2000 phospholipid. The ED50 of binding was determined by non-linear regression analysis of the binding data (“Methods” section), and converted to nM based on the 150,000 Da molecular weight of the TfRMAb.

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