Table 1 Luciferase expression (pg/mg protein) in mouse 3T3 fibroblasts at 24 or 48 h after application of THLs encapsulating the pGL4 luciferase expression plasmid DNA and targeted with either the TfRMAb or the isotype rat IgG.

From: Plasmid DNA gene therapy of the Niemann-Pick C1 mouse with transferrin receptor-targeted Trojan horse liposomes

THL storage at 4 °C (days)

TfRMAb/pGL4 THLs

Rat IgG/pGL4 THLs

24 h

48 h

24 h

48 h

0

55 ± 3

92 ± 9

 < 1

 < 1

1

66 ± 2

108 ± 7

 < 1

 < 1

5

62 ± 6

119 ± 2

 < 1

 < 1

  1. Prior to application to the 3T3 cells, the THLs were stored at 4 °C for 0, 1, or 5 days. Data are mean ± SD (3 dishes per time point).
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