Figure 1 | Scientific Reports

Figure 1

From: 4-Phenylbutyrate ameliorates apoptotic neural cell death in Down syndrome by reducing protein aggregates

Figure 1

Establishment of trisomy-specific iPSCs and early enhanced gliogenesis in trisomy 21 NPCs. (a) Schematic diagram showing the reprogramming processes of diploid control, trisomy 21, and trisomy 13 iPSCs. (b) iPSCs generated from diploid, trisomy 21, and trisomy 13 patients showed ESC-like morphology (phase image). NPCs differentiated from iPSCs expressed the NPC markers SOX2 and PAX6. Neurons differentiated from NPCs after 3 months expressed the pan-neural marker MAP2 and cortical neural marker TBR1. Scale bar = 200 μm. (c) Representative image of immunocytochemistry for a neural marker (TUJ1), glial marker (GFAP), and NPC marker (SOX1) after 3 weeks of differentiation from NPCs. Scale bar = 200 μm. (d) Quantification of the ratio of each marker-positive cell/Hoechst 33342-positive cell after 3 weeks of differentiation from NPCs. Data are presented as mean ± SEM. n = 3 per clone.

Back to article page