Figure 4 | Scientific Reports

Figure 4

From: 4-Phenylbutyrate ameliorates apoptotic neural cell death in Down syndrome by reducing protein aggregates

Figure 4

Aggregation of neurodegenerative disorder-associated proteins in trisomic neurons. (a) Representative images showing immunocytochemical staining for aggregates (red), p62 (green), and MAP2 (white) in NGN2-neurons on day 14. Scale bar = 20 μm. (b) Relative aggregate intensity per cell in NGN2-neurons (D14). *P < 0.05, **P < 0.001, n = 5–13 per clone. (c) PLA to detect the interaction between p62 and α-Syn, Parkin, or Htt in NGN2-neurons. PLA signals are seen as red dots. Hoechst 33342 counterstaining appears in blue. Scale bar = 20 μm. (d) Quantification of PLA signals (red dots) per cell. Data are presented as mean ± SEM. *P < 0.05, n = 3–6 per clone.

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