Figure 11
Co-expression of A595T and G1061E ATP7B mutants in HEK293 cells alters their subcellular distribution. HEK293 cells were co-transfected with GFP-ATP7B-A595T (green) and FLAG-ATP7B-G1061E (red) and incubated in basal medium (top panels), medium containing 100 µM CuCl2 (middle panels), or medium containing 25 µM of tetra-thiomolybdate (TTM; lower panels). Trans-golgi network (TGN) marker, TGN46, in red and nuclei (DAPI) in blue. Co-localization is indicated in yellow. The rightmost panels show × 200 magnified images. Green signals are from GFP fluorescence.
