Figure 3 | Scientific Reports

Figure 3

From: Analysis of Wilson disease mutations revealed that interactions between different ATP7B mutants modify their properties

Figure 3

Cu-transport activity of the ATP7B mutants. (A) YST Cells were transfected with the tyrosinase-encoding plasmid alone (Tyr, negative control) or together with wild-type (WT) ATP7B, the catalytically inactive D1027A mutant (negative control), or the ATP7B mutants found in WD patients. Cu-transport activity was evaluated by measuring eumelanin pigment formation (indicated with white arrows).The area (B) and intensities (C) of the pigment were quantified. (D) Total eumelanin pigment signal (area × intensity) in cells with the WD mutations were quantified and compared to the WT values. All values are reported as means ± SD (Standard deviation). Significance was determined by one-way ANOVA with Dunnet’s multiple comparison test, ****p < 0.0001, n = 3.

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