Figure 1
From: Cerebral tissue pO2 response to treadmill exercise in awake mice
(a) A custom head-plate made from titanium was glued to the skull to allow animal restraint on the treadmill. (b) The implanted titanium bar was screwed to a holder to fix the animal’s head. (c) Restrained animal on the motorized treadmill under two-photon setup. (d) Thinned skull cranial window preparation with a small thinned region next to the coverslip uncovered by dental cement (marked with black lines) for pO2 dye injection. (e–g) Representative two-photon fluorescent images of microvasculature (600 µm × 600 µm) at depths of ~ 20, 50 and 120 μm. (h)En face maximum intensity projection (MIP) of the 3D microvascular angiogram through the depths 0–150 μm.
