Figure 1

miR-669m overexpression inhibits erythroid differentiation in vivo. (A) A procedure of bone marrow (BM) transplantation is shown. BM Lin⁻ cells were transduced with pMSCV/Empty or pMSCV/miR-669m vectors. One day after the transduction, the cells were intravenously injected into recipient C57BL/6 J mice which had been irradiated with 8 Gy X-ray one day before. After 12 weeks, spleen (SPL) and BM cells were analyzed by FACS. (B, C) Representative plots (B) and pooled data (C) of CD71/TER119 expression on SPL and BM GFP⁺PI⁻ cells were described. In (C), each dot represents data from one recipient mouse (n = 6). The horizontal bars describe mean. Statistical analysis was performed using Student’s t test (*p < 0.05).