Table 2 Analysed amino acid (AA) composition (n = 1, %), pH (n = 1), volatile fatty acid (VFA) concentrations (n = 1, mmol/kg) and microbial counts (n = 2, log10 CFU/g) of the cereal fraction (CER) and the cereal fraction supplemented with xylanase and β-glucanase (CER + ENZ) collected from the fermentation tanks during the initial fermentation (0–52 h) and during the feeding trial (d10, d51).
CER | CER + ENZ | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
0 h | 12 h | 24 h | 52 h | d10 | d51 | 0 h | 12 h | 24 h | 52 h | d10 | d51 | SDa | |
AA composition | |||||||||||||
Lysine | 0.49 | 0.41 | 0.39 | 0.37 | 0.37 | 0.39 | 0.40 | 0.39 | 0.36 | 0.38 | 0.48 | 0.35 | – |
Threonine | 0.34 | 0.37 | 0.36 | 0.38 | 0.36 | 0.37 | 0.38 | 0.39 | 0.36 | 0.38 | 0.43 | 0.35 | – |
Methionine | 0.15 | 0.19 | 0.18 | 0.19 | 0.19 | 0.19 | 0.18 | 0.19 | 0.18 | 0.18 | 0.22 | 0.17 | – |
Valine | 0.46 | 0.55 | 0.53 | 0.55 | 0.53 | 0.52 | 0.51 | 0.51 | 0.50 | 0.51 | 0.60 | 0.49 | – |
Leucine | 0.65 | 0.78 | 0.75 | 0.75 | 0.73 | 0.76 | 0.76 | 0.76 | 0.75 | 0.76 | 0.86 | 0.70 | – |
Isoleucine | 0.38 | 0.41 | 0.39 | 0.41 | 0.40 | 0.40 | 0.39 | 0.38 | 0.39 | 0.39 | 0.47 | 0.36 | – |
pH | 6.34 | 6.09 | 4.47 | 3.70 | 3.87 | 3.78 | 6.31 | 6.30 | 5.43 | 3.76 | 3.92 | 3.73 | – |
VFA concentrations | |||||||||||||
Acetate | 29.55 | 16.81 | 20.23 | 27.30 | 28.09 | 50.93 | 20.96 | 16.62 | 23.88 | 23.37 | 85.14 | 84.66 | – |
Propionate | 0.64 | 0.45 | 0.30 | 0.35 | 0.30 | 0.81 | 0.54 | 0.34 | 0.36 | 0.20 | 0.42 | 1.53 | – |
Isobutyrate | 1.43 | 1.36 | 1.17 | 1.01 | 0.75 | 1.16 | 1.41 | 1.61 | 1.56 | 1.05 | 1.02 | 1.34 | – |
Butyrate | 0.51 | 0.40 | 0.23 | 0.25 | 0.15 | 0.30 | 0.47 | 0.31 | 0.25 | 0.16 | 0.31 | 0.28 | – |
Isovalerate | 0.13 | 0.12 | 0.07 | 0.08 | 0.03 | 0.08 | 0.14 | 0.13 | 0.14 | 0.07 | 0.14 | 0.08 | – |
Valerate | 0.30 | 0.19 | 0.08 | 0.10 | 0.05 | 0.14 | 0.25 | 0.17 | 0.15 | 0.06 | 0.14 | 0.17 | – |
Total VFA | 32.55 | 19.03 | 22.08 | 29.09 | 29.37 | 53.40 | 23.77 | 19.18 | 26.35 | 24.90 | 87.18 | 88.05 | – |
Acetate:Propionate | 45.88 | 37.50 | 67.32 | 78.41 | 94.19 | 63.28 | 38.59 | 48.44 | 66.63 | 115.63 | 202.20 | 55.50 | – |
Protein-derived VFAb | 1.85 | 1.66 | 1.32 | 1.19 | 0.82 | 1.37 | 1.80 | 1.91 | 1.86 | 1.17 | 1.30 | 1.58 | – |
Microbial countc | |||||||||||||
Lactic acid bacteria | 5.1 | 8.5 | 9.1 | 9.2 | 9.1 | 9.2 | 5.7 | 6.2 | 8.7 | 8.8 | 9.0 | 9.0 | 0.18 |
Enterobacteriaceae | 5.2 | 7.0 | 4.9 | < DL | < DL | < DL | 5.3 | 6.0 | 7.7 | < DL | < DL | < DL | 0.13 |
Yeasts | 4.9 | 5.0 | 5.2 | 6.8 | 6.1 | 6.3 | 3.8 | 4.2 | 5.0 | 4.8 | 6.8 | 6.7 | 0.20 |
Moulds | < DL | < DL | < DL | < DL | < DL | < DL | < DL | < DL | < DL | < DL | < DL | < DL | – |