Figure 1
From: RLP23 is required for Arabidopsis immunity against the grey mould pathogen Botrytis cinerea
The Arabidopsis rlp23 mutations enhanced the susceptibility to B. cinerea. 4–5-week-old plants were inoculated with 5 µl of conidial suspensions (1 × 105 conidia/mL) of B. cinerea. (A) Lesion development on each mutant. The photograph was acquired at 7 dpi. (B) Lesion areas were measured at 7 dpi. At least 24 lesions were measured from each line. The statistical significance of differences in lesion size was determined by Tukey’s honestly significant difference (HSD) test (**P < 0.01). The experiment was repeated three times, with similar results. (C) Growth of B. cinerea in planta based on qPCR using B. cinerea- and Arabidopsis-specific primers. BcCutA was quantified by qPCR in the extracted genomic DNA. The Arabidopsis ⍺-shaggy kinase gene (AtASK) was used as a reference. Means and SDs were calculated from three independent samples. The statistical analysis was conducted using two-tailed Student’s t-tests. The level of BcCutA was compared at the same time points between Col-0 and the rlp23-2 mutant plants (*P < 0.05).
