Figure 6
From: RLP23 is required for Arabidopsis immunity against the grey mould pathogen Botrytis cinerea
The Arabidopsis rlp23 mutation did not enhance the susceptibility to A. brassicicola. (A) A conidial suspension (5 × 105 conidia/mL) of A. brassicicola was spray-inoculated onto 4–5-week-old Col-0 plants. AbNLP1 was quantified by RT–qPCR. AbEF1 was used as internal control. Means and SDs were calculated from three independent samples. AbNLP1 was only detected at 24, 48 and 72 hpi in Arabidopsis Col-0 plants. (B) Leaves from 4–5-week-old plants were drop-inoculated with 5 µl of conidial suspensions (1 × 105 conidia/mL) of A. brassicicola. The susceptibility to A. brassicicola was not affected in Arabidopsis rlp23-1 and rlp23-2 mutants compared with Col-0 plants, whereas the cyp71A12 cyp71A13 mutant exhibited enhanced susceptibility to this pathogen. The photograph was taken at 4 dpi. (C) Lesion areas were measured in the experiments (B). At least 40 lesions from each line were measured at 4 dpi. The statistical significance of differences in lesion size was determined by Tukey’s honestly significant difference (HSD) test (**P < 0.01). The experiment was repeated twice, with similar results.
