Figure 5
From: Deletion of DJ-1 in rats affects protein abundance and mitochondrial function at the synapse
Bioenergetic analysis of striatal synaptic mitochondria from DJ-1 KO rats. Mitochondrial OCR was measured in striatal synaptic mitochondria isolated from 3-month-old WT and DJ-1 KO rats using a Seahorse XF24 analyzer (n = 3). (A) Electron flow assay assessing complex I- (pyruvate/malate), II- (succinate), and IV- (ascorbate/TMPD) driven respiration. Indicated are the mean ± SEM values. Two-way ANOVA revealed a significant effect of genotype (p < 0.0072). (B) Coupling assay assessing succinate-driven respiration. Mitochondria were administrated sequential injections of ADP, oligomycin, FCCP, and rotenone/antimycin A. Two-way ANOVA revealed a significant effect of genotype (p < 0.0003). Sidak’s post-hoc comparison test revealed FCCP, a depolarizing agent inducing maximal respiration, to be significantly increased in DJ-1 KO rats (indicated by *p < 0.05).
