Figure 2
Stable integration, expression and yield of the bovine lysozyme (BvLzm) recombinant gene in sugarcane BvLzm transgenic lines as determined by Southern (a) and northern (b) blot analyses and enzyme-linked immunosorbent assay (ELISA) (c), respectively. Representative lines with single or multiple promoter:BvLzm-terminator cassettes are shown. BvLzm, maize codon-optimized BvLz; pU:BvLzm, BvLzm driven by the maize ubiquitin 1 promoter (pU); pUD:BvLzm, BvLzm expressed from two promoters, pU and sugarcane dirigent16 (pD); pUDE:BvLzm, BvLzm expressed from three promoters, pU, pD and sugarcane elongation factor 1α (pE); and pUPBE:BvLzm, BvLzm expressed from four promoters, pU, sugarcane proline-rich protein (pP), Sugarcane bacilliform virus (pB) and pE. DNA and RNA gel blots were hybridized to a probe corresponding to the coding region of BvLzm. The full-length uncropped DNA and RNA gel blot autoradiograms are displayed in Supplementary Figures S2 and S3, respectively. The BvLzm yield is indicated as determined by ELISA in juice extract of culms (1.0 kg of culm).
