Figure 5
Enhancement of culm biomass and yield of recombinant bovine lysozyme (BvLzm) by fertilization in triple promoter:BvLzm sugarcane lines. BvLzm activity of four representative lines is shown as determined by enzyme-linked immunosorbent assay in juice extract of culms (1.0 kg of culm). Values represented four biological samples and three technical replications at 2, 6 and 8 months following low (LF) or high (HF) fertilization. Values with different letters are significantly different (p < 0.05). BvLzm, maize codon-optimized BvLz; 32C, pUPE:BvLzm line; 18, 44 and 54, pUDE:BvLzm lines; U, maize ubiquitin 1 promoter; P, sugarcane proline-rich protein promoter; E, sugarcane elongation factor 1α promoter; and D, sugarcane dirigent16 promoter.
