Figure 1 | Scientific Reports

Figure 1

From: CRISPR/Cas12a mediated knock-in of the Polled Celtic variant to produce a polled genotype in dairy cattle

Figure 1

Schematic depiction of the horned locus adapted from Wiedemar et al.7. The Polled Celtic (Pc) variant (bottom) consists of a 208 bp duplication in combination with a 6 bp deletion. The CRISPR/Cas12a system targeted the 6 bp sequence of the wild-type (WT) variant (red bar) which is not present in the Pc variant. Specific primers (btHP-F1 and btHP-R2) were employed to distinguish between the WT sequence (389 bp) and the Pc variant (591 bp).

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