Figure 1 | Scientific Reports

Figure 1

From: Molecular characterization of pyridoxine 5′-phosphate oxidase and its pathogenic forms associated with neonatal epileptic encephalopathy

Figure 1

Physical properties of PNPO variant forms. Only results from the wild type (black lines), G118R (red lines) and X262Q (blue lines) PNPO enzymes are shown. All the other variants have the same characteristics as the wild type enzyme and therefore are not reported. (a) Size exclusion chromatography analyses on PNPO variants. Elution profiles were obtained in 20 mM potassium-phosphate buffer, pH 7.6, containing 150 mM NaCl and 5 mM 2-mercaptoethanol. Far-UV CD (b) and absorption (c) spectra of wild type, G118R and X262Q PNPO enzymes are shown. Inset in panel C is an expanded view of the visible region of the same absorption spectra, showing absorption bands due to FMN. For each protein sample three independent preparations were analysed, although in the figure a single spectrum is shown of wild type (71% saturation with respect to FMN), G118R (30% saturation) and X262Q (10% saturation). All spectra were measured in 20 mM potassium-phosphate buffer, pH 7.6. Images were generated using the software Prism 8 (GraphPad; https://www.graphpad.com/scientific-software/prism/).

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