Figure 2 | Scientific Reports

Figure 2

From: Testing the impact of a single nucleotide polymorphism in a Plasmodium berghei ApiAP2 transcription factor on experimental cerebral malaria in mice

Figure 2

S1823F mutation in ApiAP2 TF of PbNK65 does not induce experimental cerebral malaria. (A, B) Mice were inoculated with WT PbNK65S, mutant PbNK65F or WT PbANKAF iRBCs as in Fig. 1 and brains were collected from infected mice and uninfected controls at various time points. Sites of brain hemorrhage are shown as patchy dark blue colorations on brain photos of Evans blue injected mice. Photos are representative of more than 10 mice per group (A). (B) Tissue sections taken from different parts of infected mouse brains were stained with Hematoxylin and Eosin and then evaluated under light microscopy for histological signs of brain pathology. Sites of hemorrhage in 10 × magnified slides are shown with black arrow heads. RBC congested areas and signs of edema are shown in 40 × magnified slides with green and blue arrow heads respectively. 3 mice/group/day were used for collecting tissue sections.

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