Figure 2 | Scientific Reports

Figure 2

From: The methylation status of the chemerin promoter region located from − 252 to + 258 bp regulates constitutive but not acute-phase cytokine-inducible chemerin expression levels

Figure 2

Adipocytes are key cells expressing chemerin following stimulation with IL-1β + OSM. eWAT or SCA-1 + APs were isolated. The cells were differentiated to obtain a mature adipocyte cell culture. Then, the cells were treated with IL-1β (10 ng/mL), OSM (50 ng/mL), or a combination for up to five days. The levels of chemerin mRNA (A, C) were determined using RT-QPCR. The relative expression of stimulated cells over the control is shown. Levels of secreted chemerin were determined in parallel in conditioned media by ELISA (B, D). Data are presented as the mean ± SEM of at least three independent experiments. Statistical significance between the control and the treated cells is indicated by an asterisk; *p < 0.05 by analysis of covariance followed by a Bonferroni post-hoc test.

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