Figure 3 | Scientific Reports

Figure 3

From: The methylation status of the chemerin promoter region located from − 252 to + 258 bp regulates constitutive but not acute-phase cytokine-inducible chemerin expression levels

Figure 3

Chemerin transcription is linked to the DNA methylation status of the RARRES2 promoter region from − 735 to + 258 bp. Primary mouse hepatocytes and mature adipocytes derived from sorted APs were treated with IL-1β (10 ng/mL) and OSM (50 ng/mL) for 48 h. B-cells, which do not produce chemerin, were adopted as a control. The DNA methylation status of the RARRES2 promoter sequence was analyzed by bisulfite sequencing. The localization of CpG sites (A) and the DNA methylation levels of each CpG site from − 735 to + 258 bp of the RARRES2 promoter are shown (B). The differences in the average methylation level of the proximal (C) and distal (D) parts of the promoter were analyzed using the nonparametric Kruskal–Wallis test; *p < 0.05. Data are presented as the mean ± SEM of at least three independent experiments.

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