Figure 6

Genes of cytokines/chemokines and receptors differentially expressed in trigeminal ganglia-resident HSV-specific CD8⁺ T cells from HSV-1 infected symptomatic vs. asymptomatic HLA Tg rabbits. (a) Expression of genes of chemokines and chemokine receptors detected by single-cell RNA sequencing from TG-resident HSV-specific CD8⁺ T cells from SYMP vs. ASYMP HLA Tg rabbits is represented through t-SNE plots (top panels). Expression of genes of cytokines and cytokine receptors detected by single-cell RNA sequencing from TG-resident HSV-specific CD8⁺ T cells from SYMP vs. ASYMP HLA Tg rabbits is represented through t-SNE plots (lower panels). (b) Average frequencies of CD8⁺ T_RM cells expressing genes of cytokines/chemokines and receptors in TG of HSV-1 infected SYMP vs. ASYMP HLA Tg rabbits. (c) Bulk RNA sequencing showing expression of genes of chemokines/chemokine receptors (left panel) and genes of cytokines/cytokine receptors (right panel) in HSV-specific T_RM cells from SYMP vs. ASYMP HLA Tg rabbits. (d) Representative (left panels) and average (right panels) frequencies of CCR3⁺CD8⁺ T_{RM cells} in TG of HSV-1 infected SYMP vs. ASYMP HLA Tg rabbits. (e) Fluorescence microscopy images showing infiltration of HSV-1 infected TG from SYMP vs. ASYMP HLA Tg rabbits by CD8⁺ T cells expressing T cell-attracting chemokines and receptors (i.e., CXCR3, CXCL9, CXCL10, and CXCL11) (magnification, × 20). Blue: DAPI (DNA stain); red: CXCR3 cells.