Figure 2
From: Evidence of genetic isolation between two Mediterranean morphotypes of Parazoanthus axinellae
Haplotype network analysis for COI (above) and ITS (below) gene markers in Parazoanthus axinellae species complex. Haplotype network was built in Rstudio version 1.1.453 (https://rstudio.com/) with an R package pegas (https://cran.r-project.org/web/packages/pegas/index.html), using an infinite site model based on Hamming distances of DNA sequences. Each haplotype is presented as a separate pie chart, and size of the pie charts directly correlates to the number of individuals within the haplotype. Pie chart colors for geographic location were chosen to intuitively associate the reader to Parazoanthus axinellae morphotypes, with orange tones being attributed to the “Stocky” morphotype, and yellow and grey tones to the “Slender” morphotype. Distances between haplotypes correspond to the genetic differentiation observed for each of the markers, and each mutation between the haplotypes is shown as a hyphen. Alluvial diagrams were made using an online platform RAWGraphs (https://rawgraphs.io/) to further clarify haplotype-morphotype assignments, and final graph modifications were done in Inkscape version 0.92.4 (https://inkscape.org/it/).
