Figure 5
WDR13 interaction with ERα and its effect on ERα transcriptional activity. (a) WDR13 represses ERE reporter assay in both presence and absence of estradiol though in presence of TSA, the repression was removed. (b) WDR13S (Cy3, red) colocalizes with ERα (GFP, green). (c) Interaction of WDR13S with ERα is independent of nuclear receptor box motif wherein the anti-FLAG agarose beads pulled down ERα in both intact and mutated WDR13S overexpressing cells. (d) WDR13 interacts with CDEF domain of ERα (as indicated by presence of 49.5 kDa band) as well with whole ERα (as indicated by presence of 63.8 kDa band) on pull down with anti-FLAG agarose beads. (e) WDR13 does not interact with ABC domain of ERα (as indicated by lack of interaction with 37.4 kDa band). The values indicated by asterisk (*) differ significantly at p < 0.05. The experiments were repeated twice.
