Figure 1

Generation of a mouse model of spontaneous breast cancer bone metastasis by in vivo selection. (A) Schematic summary: a model of spontaneous breast cancer bone metastasis generated by in vivo selection. Two cycles were performed, creating 4T1.1 and 4T-Bone clones. (B) Tumor growth curve of the different clones; n = 7 mice per group. Error bars represent SEM. (C) Tumor weight of the resected primary tumors. Error bars represent SEM. Kruskal–Wallis test. (D) Kaplan–Meyer survival curve of mice injected with the three clones; 4T1: n = 10, 4T1.1: n = 11, 4T-Bone: n = 9. Log-rank (Kruskal–Wox) test. (E) Representative μCT image of spinal metastasis. (F) Bone metastasis burden of the different clones, Kruskal–Wallis test. (G) Quantification of bone metastatic area in mice injected with bone-tropic cells. Welch's t-test. n = 7 (4T1), n = 6 (4T1.1), n = 7 (4T-Bone) for (F&G). (H) Representative images of H&E sections of bone metastasis. Left, middle: coronal view of spinal column w/wo bone metastasis. Right: transversal view of a vertebra. (I) Metastatic incidence in lungs, lymph nodes and bones in 4T1 or 4T-Bone injected mice at day 40. n = 6 per group.