Figure 4

Immunosuppression in the BM precedes bone metastasis. (A) Scheme of the experimental design of early/pre-metastatic stage (day 16) analysis. (B) Quantification of the CD45⁺CD11b⁺CD11c⁻ total myeloid cell population. (C) Quantification of CD45⁺CD11c⁻CD11b⁺Ly6G⁺Ly6C^int granulocytes. (D) Quantification of CD45⁺CD11c⁻CD11b⁺Ly6G⁻Ly6C⁺ monocytes. (E) Quantification of CD45⁺CD11b⁻CD11c⁺ dendritic cells. (F) Quantification of CD45⁺CD3⁻B220⁺ B cells. (G) Quantification of CD45⁺B220⁻CD3⁺ T cells. (H) Quantification of CD45⁺B220⁻CD3⁻Nkp46⁺ NK cells. Number of mice per each group is specified in graphs. Data are presented as % of CD45, normalized to the average of normal group. Error bars represent SEM. p < 0.05; Kruskal–Wallis multiple comparison test.