Figure 1
From: Diverse species-specific phenotypic consequences of loss of function sorting nexin 14 mutations
Generation of Snx14 mutant mice. (A) The C57BL/6J mouse Snx14 gene was targeted with sgRNA guided CRISPR/Cas9 to cut specific sites. Flags display the position of these sites (Chromosome 9) either side of exon 3. The selected mutant had a 571-nucleotide deletion encompassing exon 3 of the Snx14 gene, resulting in a frame shift K114fs + 5*. (B) Primers (arrow heads) flanking this deletion were used to examine the mutation yielding bands of 982 bp from the Snx14WT/WT allele and 411 bp in from the Snx14KO allele. (C) PCR products generated from the primers flanking the deleted region. (D) Western blot showing loss of SNX14 protein in Snx14KO/KO mice. Original gels and blots are presented in Supplemental Fig. S2.
