Figure 5
Inducible wnd gain-of-function alleles antagonize the effects of ectopic Alk expression. (A) Schematic representation of the wnd gene structure according to FlyBase (version FB2019_05). Black boxes represent protein-coding exons, grey boxes UTRs; lines indicate intronic regions of the four predicted wnd-transcripts. The positions of characterized point mutations and P-element insertions are highlighted. P{XP}d00622 and P{XP-U}Exel6135 (wndd00622 and wndExel6135) contain UAS-elements (direction indicated by arrows) and are inserted in or immediately upstream of the wnd 5′UTR. P{XP-U}Exel6135 is a hybrid P-element flanking the chromosomal deficiency Df(3L)Exel6135. Scale bar = 1 kb. (B–E, J–Q) Eyes of adult female flies with the indicated genotypes are shown. n > 100 animals, 100% penetrance. Scale bar is 100 µm. (F–I) To visualize ommatidia patterns in adult female Drosophila eyes, eye sections were stained with toluidine blue. (F,G) sevEP-Gal4/ + and sevEP-Gal4 > Wnd/ + controls. (H) Visible necrotic scars and absence of ommatidia in the anterior part of an eye upon sevEP-Gal4/ + induced UAS-Alk/ + expression. (I) Overexpression of Wnd/ + rescues ommatidia patterns in sevEP > UAS-Alk flies (H). Scale bar = 20 µm.
