Figure 2
From: Tissue-targeted R-spondin mimetics for liver regeneration
Confirmation of receptor-specific activation of Wnt signaling. (A) STF activity in HEK293 cells transiently transfected with TFR1 (control, top), ASGR1 (middle), and ASGR1 together with ASGR2 cDNAs (bottom), either in the presence (left) or absence (right) of exogenous Wnt source (30% Wnt3a conditioned media). (B) STF activity in A431 cells with TFR1 or ASGR1 overexpression as specified. (C) Flow cytometry analysis of cell surface level of FZD proteins. HEK293 cells were transiently transfected with either ZNRF3 alone (top) or ASGR1 and ZNRF3 cDNAs (bottom), treated with RSPO mimetics as specified at 10 nM, then stained by the pan-FZD antibody 18R5.
