Figure 6
From: Neurotoxicity of HIV-1 Tat is attributed to its penetrating property
Relative Tat levels in exosomes or brain by ELISA. (A) Exosomes were isolated from mouse plasma by differential ultracentrifugation. IP lysis buffer (Pierce) was used to release proteins from exosomes. Tat levels were determined using a modified ELISA protocol. (B) Mouse brain was homogenized in IP lysis buffer to prepare brain lysate. One hundred μL was used for Tat ELISA analysis. Note: n = 3, p < 0.05 indicates statistically significant.
