Figure 1
Cisplatin induces a senescence-like response in DRG neurons. (a) Representative images of SA-β-gal stained primary DRG neurons cultured from WT C57BL/6 mice. Percent SA-β-gal positive cells was calculated as the number of neurons with positive SA-β-gal staining divided by the total number of cells left on the coverslip times 100 (n = 3). P = 0.0497. (b) p16INK4A was detected by anti-p16INK4A immunofluorescence (red). Percent cytosolic p16INK4A positive cells was calculated as the number of neurons with cytosolic p16INK4A immunofluorescence, visualized by DAPI nuclei staining (blue), divided by the total number of cells left on the coverslip times 100 (n = 3). P = 0.0113. (c) Visualization of HMGB1 as detected by anti-HMGB1 immunofluorescence (red). Percent cytosolic HMGB1 positive cells was calculated as the number of neurons with cytosolic HMGB1 immunofluorescence divided by the total number of cells left on the coverslip times 100 (n = 3). Data points are mean values ± SEM and were analyzed by two-tailed Student’s t-test.
