Figure 3
Genetic clearance of senescent cells reverses CIPN in mice. (a) Cisplatin and GCV treatment regimen to induce CIPN and eliminate senescent cells, respectively, in p16-3MR mice. (b, c) Response of p16-3MR mice, which are sensitive to GCV-mediated senescent cell clearance, to (b) mechanical and (c) thermal stimulation before and after cisplatin treatment. Cisplatin-treated mice also underwent senescent cell clearance via GCV treatment. Mechanical sensitivity of cisplatin-treated mice was compared to saline-treated (P = 0.0091, P < 0.0001, and P = 0.0077) and cisplatin-treated mice following senescent cell removal (P = 0.0007 and 0.0052). Thermal sensitivity of cisplatin-treated mice was also compared to saline-treated (P = 0.0323, 0.0084, and 0.0128) and cisplatin-treated mice treated with GCV (P = 0.0255). Data points are mean values ± SEM and were analyzed by two-way ANOVA analysis with post-hoc Tukey test. n = 6.
