Figure 3
From: MicroRNA-107 enhances radiosensitivity by suppressing granulin in PC-3 prostate cancer cells
Knockdown of GRN enhanced radiosensitivity of PC-3 cells. (a) GRN expression was repressed by shRNAs at the mRNA level. qRT-PCR was conducted to quantify GRN expression after transfection with shRNAs into PC-3 cells. (b) GRN expression was repressed by shRNAs at the protein level. Western blotting was performed after transfection of PC3 cells with shRNAs. (c) Cell proliferation and (d) colony formation of PC3 cells transfected with shGRN or scramble shRNA after exposure to 8 Gy IR. Data were representative of more than three independent experiments, with each performed in triplicate. (*p < 0.05; **p < 0.01).
