Figure 4

GRN overexpression (O/E) rescued proliferation and neutralized radiosensitivity enhanced by miR-107 mimic in PC-3 cells. Relative expression of GRN mRNA (a) and protein (b) after cotransfection with NC mimic + pcDNA3.1, miR-107 mimic + pcDNA3.1, NC mimic + pcDNA3.1-GRN, or miR-107 mimic + pcDNA3.1-GRN, respectively. (c) Cell proliferation and (d) colony formation of PC-3 cells cotransfected with the above RNAs and plasmids after IR. Data were representative of more than three independent experiments, with each performed in triplicate. (*p < 0.05; **p < 0.01). pcDNA3.1 represents the empty vector pcDNA™3.1⁽⁻⁾. pcDNA3.1-GRN represents the vector pcDNA™3.1⁽⁻⁾ containing the GRN gene construct. NS represents the none significant.