Figure 1 | Scientific Reports

Figure 1

From: Improved locomotor recovery after contusive spinal cord injury in Bmal1−/− mice is associated with protection of the blood spinal cord barrier

Figure 1

Acute dysregulation of the clock pathway in the contused spinal cord. WT female mice were subject to SCI (T9 IH contusion, 50 kdyn) or sham surgery and sacrificed at the indicated times. Total RNA (a) or proteins (b, c) were extracted from a 5 mm spinal cord segment that included the injury epicenter and the penumbra. (a) Expression of the clock pathway genes was analyzed by qPCR. Sham controls sacrificed at 6 (n = 2) or 24 h (n = 3) were pooled together as they showed little variability for the tested Clock pathway transcripts. After SCI, Bmal1 and its target genes Cry1 and Per1 were upregulated. However, two other BMAL1 target genes Dbp1 and Nr1d1 showed an opposite trend or no change, respectively. (b, c) Western blot analysis revealed reduced levels of BMAL1 protein. Untrimmed western blot images are shown in Supplementary Fig. S10. Data are mean ± SD (*p < 0.05; ns, p > 0.05, u-test).

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