Figure 3

Expression of BMAL1 in grey matter cells. Animals, co-immunostaining and imaging were as described for Fig. 2. (a) Representative images of BMAL1 signal in the ventral horn grey matter and the ventrolateral white matter after co-staining for the neuronal marker NeuN. The sectioning level is indicated by the red line. The position of higher power images that are shown in (b) is indicated. (b) High power images of BMAL⁺ grey matter cells that were co-stained for the neuronal marker NeuN, the astrocyte marker GFAP and the EC marker PECAM1. Arrows point double positive cells. All co-immunostainings were done on adjacent sections that were cut as in (a); low power images are shown in Supplementary Figs. S2–S5.