Figure 2
From: Evolution of isoprenyl diphosphate synthase-like terpene synthases in fungi
In vitro IDS enzymatic assays of the GGDPSs and ILTPSs. (A) Assays of MlpGGDPS (P), MlpILTPS1 (PIL1), MlpILTPS2 (PIL2) from Melampsora larici-populina using DMADP, GDP, and FDP together with [1-14C]-IDP as substrates. (B) Assays of MliGGDPS (I), MliILTPS1 (IIL1), MliILTPS2 (IIL2) and MliILTPS3 (IIL3) from Melampsora lini using DMADP, GDP, and FDP together with [1-14C]-IDP as substrates. In both (A) and (B), the reaction products were separated via thin-layer chromatography (TLC). CsaLSU/CsaSSU I from cucumber was used as a positive control (P.C.). Boiled CsaLSU/CsaSSU I was used as a negative control (N.C.). GOH geraniol, FOH farnesol, GGOH geranylgeraniol. The final products were determined by phosphor-imaging and displayed with ImageJ software. Full-length blots are presented in Fig. S3.
