Figure 3
From: Evolution of isoprenyl diphosphate synthase-like terpene synthases in fungi
GC chromatograms of the terpene synthase enzyme assays. (A) In vitro monoterpene products formed from recombinant MlpGGDPS and MlpILTPSs using GDP as substrate. (B) In vitro monoterpene products formed from recombinant MliGGDPSs and MliILTPSs using GDP as substrate. (C) In vitro sesquiterpene products formed from recombinant MlpGGDPS and MlpILTPSs using FDP as substrate. (D) In vitro sesquiterpene products formed from recombinant MliGGDPSs and MliILTPSs using FDP as substrate. All products were analyzed by gas chromatography-mass spectrometry (GC–MS), and the total ion chromatograms (TIC) are shown. 1, (E)-β-ocimene; 2, linalool; 3, (E,E)-α-farnesene; 4, (E)-nerolidol. (E) Structures of the two monoterpene and two sesquiterpene products. Linalool and (E)-nerolidol were identifed using authentic standards (Fig. S4). (E)-β-ocimene and (E,E)-α-farnesene were identified based on comparison of their respective Kovats retention index (Table S2).
