Figure 4
From: Evolution of isoprenyl diphosphate synthase-like terpene synthases in fungi
Key amino acids for terpene synthase activities of ILTPSs. (A) Homology-based structural model of MliGGDPS (red) and MliILTPS2 (blue). Shown are three amino acids in the active sites that differ between GGDPS and ILTPSs. Numbering of residues according to MliGGDPS. (B) Volatiles from the headspace of E. coli cultures expression recombinant MliGGDPS R81P. (C) Volatiles from the headspace of E. coli cultures expression recombinant MliGGDPS D250N/N254S. (D) Homology-based structural model of the (E,E)-α-farnesene synthase MliILTPS2 (blue) and the (E)-nerolidol synthase MliILTPS3 (sand). Shown is one amino acid in the active sites that differ between MliILTPS2 and MliILTPS3. The positon of the amino acid is numbered according to MliILTPS2. (E) Volatiles from the headspace of E. coli cultures expression recombinant MliILTPS3 S73C. (F) In vitro sesquiterpene products formed from recombinant MliILTPS3 S73C using FDP as substrate. 3, (E,E)-α-farnesene; 4, (E)-nerolidol. IC internal compound indole.
