Figure 1 | Scientific Reports

Figure 1

From: Donor myeloid derived suppressor cells (MDSCs) prolong allogeneic cardiac graft survival through programming of recipient myeloid cells in vivo

Figure 1

Donor-derived MDSCs suppress alloimmune reaction in vitro and in vivo. (A) Naïve C57BL/6T cells were stimulated with BALB/c antigen presenting cells (APCs: BALB/c bone marrow derived cDCs). MDSCs or cMDCs were added as modulator. CD4+ and CD8+ T cell proliferation in response to allogeneic cDCs was analyzed by CellTrace violet dye dilution (n = 5 per group). (B) Graphs showing the proliferation of CD4+ and CD8+ T cells in the presence of MDSCs compared to cMDCs. Mean ± SEM, *p < 0.05, **p < 0.01, two-tailed unpaired t test. Data represents one of 4 separate experiments. (C) Schematic diagram of the experimental design. C57BL/6 recipients received a single-dose intravenous injection of 1 × 106 BALB/c MDSCs or cMDCs 7 days prior to the cardiac transplantation. (D) Kaplan–Meier cumulative survival of allograft shows the prolonged survival in MDSCs treated group compared to control groups (n = 8–9 per group). **p < 0.01, ***p < 0.001, log-rank test.

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