Figure 1 | Scientific Reports

Figure 1

From: Donor myeloid derived suppressor cells (MDSCs) prolong allogeneic cardiac graft survival through programming of recipient myeloid cells in vivo

Figure 1The alternative text for this image may have been generated using AI.

Donor-derived MDSCs suppress alloimmune reaction in vitro and in vivo. (A) Naïve C57BL/6T cells were stimulated with BALB/c antigen presenting cells (APCs: BALB/c bone marrow derived cDCs). MDSCs or cMDCs were added as modulator. CD4+ and CD8+ T cell proliferation in response to allogeneic cDCs was analyzed by CellTrace violet dye dilution (n = 5 per group). (B) Graphs showing the proliferation of CD4+ and CD8+ T cells in the presence of MDSCs compared to cMDCs. Mean ± SEM, *p < 0.05, **p < 0.01, two-tailed unpaired t test. Data represents one of 4 separate experiments. (C) Schematic diagram of the experimental design. C57BL/6 recipients received a single-dose intravenous injection of 1 × 106 BALB/c MDSCs or cMDCs 7 days prior to the cardiac transplantation. (D) Kaplan–Meier cumulative survival of allograft shows the prolonged survival in MDSCs treated group compared to control groups (n = 8–9 per group). **p < 0.01, ***p < 0.001, log-rank test.

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